A method to study bacterioplankton community structure in Antarctic freshwater lake samples is described. Small samples (between 300 and 1000 ml) taken in remote field locations were used for crude DNA extraction, followed by PCR amplification of 16S rRNA gene fragments using group-specific primers. The amplification products of the PCR reaction were then separated using denaturing gradient gel electrophoresis to produce a profile of the bacterioplankton community. Whilst the technique is only semi-quantitative, it readily differentiated communities from lakes of different trophic status and from vertical profiles within different lake types. The method offers a sensitive tool for screening and monitoring Antarctic freshwater environments as a precursor and adjunct to more detailed studies.
× HOBOKEN — Union City resident and local Hoboken hero Erik Warg died Friday, Oct. 6 at age 37.In September 2014, Warg was commended by the city of Hoboken and Mayor Dawn Zimmer for saving a 38-year-old woman from drowning in the Hudson River after she leapt into the water to reportedly get a stuffed animal. He and another person dining nearby, Thomas Licciardi, jumped in to help retrieve her from the water.In 2004, Erik graduated from West Virginia University with his Bachelor of Science degree and worked on Wall Street. Most recently he was the National Sales Director of DEVTRIBE Software and he was a volunteer for State Sen. Brian P. Stack in Jersey City.He also gave his time and talents to several causes that involved children, education, economic empowerment, arts and culture and civil rights and social action.He is survived by his two sons, Kaine C. and Kruz H. Warg; his brother, Kristoffer D. Warg and his wife, Annemarie; and his nephew, Jake. He also is survived by his Uncle Richard A. Warg and his wife, Judy; his godmother, Aunt Sarmite Judson and her husband, Bill; and several cousins, Lara Melon and her husband, James, Robert Judson and his wife, Andrea, Suzanne Warg-Holmes and her husband, Chris, Richard Warg Jr. and his wife, Jill, and Shawn Warg and his wife, Shelly.Erik’s family will receive condolences from 9:30 a.m. until the start of his Celebration of Life service at noon on Thursday, Oct. 12, at the Davisville Church, 325 Street Rd., Southampton, PA 18966.Contributions in his name may be made to the Warg Children Educational Fund c/o First National Bank of Newtown, 832 Second Street Pike, Richboro, PA 18954, Attn.: Karen Crawford. www.fluehr.com.
Lewis has hypoplastic left heart syndrome, an incurable condition. He had his first operation just four years old and his heart was so swollen that his chest couldn’t be closed for 11 days. He then had major surgery at eight months old, and again in May last year. And during this treatment, it was also discovered that one of Lewis’ lungs was not working.Thanks to Make A Wish, Lewis and his family were treated to a trip to Disneryland where he was able to star in his own super hero movie. You can watch him on his trip and explaining his condition here:Bakers, YOU can help other young people like Lewis by signing up here, plus it also gives YOU the chance to promote YOUR craft to the wider public. During the week there will be tastings, promotions, competitions and fundraising activities for Make-A-Wish.Here at British Baker we want to know what you have planned for the week. Email us via [email protected] or tweet via @BritishBaker Here is a heart-warming video which shows why the industry should support Craft Bakers’ Week.In the clip below you see seven year old Lewis, who was born with half a heart, learn that his dream of becoming a super hero for the day had been granted by Make A Wish.This year, Craft Bakers’ Week is again joining up with Make A Wish to help dreams like this come true for children and young people who are fighting life threatening conditions.
The days of the spray-painted off-white microphone are long gone. Barbra Streisand released her latest album—titled Partners—on September 15. The set list features an assortment of duets with Babs and various recording artists, from Blake Shelton to Michael Bublé to the late Elvis Presley. To celebrate the album drop, the legendary diva stopped by The Tonight Show, in her first appearance on the NBC staple since 1963 (back when Johnny Carson was host), and provided a taste of some of the tracks. And since Shelton, Bublé and (obviously) Presley weren’t around, who better to step in than Jimmy Fallon himself? The two rocked out to “Love Me Tender,” “I’d Want It to Be You” and “It Had to Be You.” Will Fallon appear on Babs’ next duets album? We definitely wouldn’t mind. Perhaps “Small World” from Gypsy? View Comments
About the Artist: With a desire to celebrate the magic of live theater and those who create it, and with a deep reverence for such touchstones as the work of Al Hirschfeld and the wall at Sardi’s, Squigs is happy and grateful to be among those carrying on the traditions where theater and caricature meet. He was born and raised in Oregon, lived in Los Angeles for quite a long time and now calls New York City his home. Lupita Nyong’o plays The Girl amid the chaos of the Liberian Civil War in Eclipsed, which opens officially on Broadway on March 6. Directed by Liesl Tommy, the production, which follows the captive wives of a rebel officer, is playing at the John Golden Theatre. To celebrate the Broadway premiere of Danai Gurira’s play, Broadway.com resident artist Justin “Squigs” Robertson penned this sketch of the Oscar winner alongside Saycon Sengbloh as Helena, Pascale Armand as Bessie, Akosua Busia as Rita and Zainab Jah as Maima.Broadway.com wishes all involved a happy opening! © Justin “Squigs” Robertson View Comments Eclipsed Related Shows Show Closed This production ended its run on June 19, 2016
Athens, Ga. – U.S. Secretary of Agriculture Ann Veneman told farmers and farm policy makers here that the United States must embrace freer trade with foreign countries “or our farmers will be left behind.”Veneman gave the keynote address at the third annual Symposium on the Future of American Agriculture Southern Region Thursday. “We have to pursue trade agreements that allow U.S. agriculture to compete across the world,” she said.The United States has to start and take part in more trade negotiations with other countries and expand into markets outside its borders, she said. Because of efficient farming practices and technologies, U.S. farmers can produce far more than the domestic markets demand.Because the United States dragged its feet during trade negotiations with Chile, she said, U.S. farmers are losing market shares to Canada, which has established trading ties with Chile.Foreign Markets ExpandingPopulation growth in Russia and in African and Asian countries will surge, she said. More than 600 million middle-class people in these countries will be “eager to spend more on better foods.”U.S. farmers should be allowed to supply that food.American agricultural exports have doubled over the past 15 years, she said, totaling about $1 billion every week. And domestic farm income has become more dependent on foreign trade in recent years. Agricultural exports account for 30 percent of U.S. farm receipts.Ongoing efforts in research and technology can allow U.S. farmers to enhance yields and crop production, “feed an expanding world population and maintain a critical role in meeting domestic and world food needs,” she said.Veneman said the administration has not taken a position concerning the current state of quota-based programs, such as peanuts and tobacco, two major programs for Georgia agriculture. The quota peanut program could be phased out over the next 5 years.Funds for Rural GeorgiaShe also announced that $3.65 million in loans and grants will be coming to meet business, housing, electric and wastewater infrastructure needs in rural Georgia.The U.S. House of Representatives recently passed H.R. 2646, its proposed version of the 2002 Farm Bill. Experts compared the current farm bill to the House’s proposed version.Economists said without changes to the current bill, many U.S. farmers will continue to face serious cash flow problems.Abner Womack, a Texas A&M economist, said the future farm bill will have to be flexible. “When things are bad, it puts money on the table,” he said. “When times are good, it puts less.”House Version of Farm BillThe House’s proposed farm bill is not a complete remedy for the current farm crisis in the United States, said Ed Smith, also with Texas A&M University. But it does provide a chance for better farm cash flow in most cases.U.S. Senator Zell Miller (D-Ga.) said the current farm bill has failed to deliver adequate safety measures for U.S. farmers. “We must drastically change our current program,” he said.The nation will have to answer one question relating to any future farm policy, he said: “Do we want our food supply produced domestically, or do we want to import it? We’re at a crossroads in American farm policy.”The annual symposium is hosted by the University of Georgia College of Agricultural and Environmental Sciences. Photo: Brad Haire Ann VenemanU.S. Secretary of Agriculture
The Nicaraguan Army reinforced its vigilance on the border with Costa Rica in order to prevent drug-trafficking activity and protect the natural reserves in the area and the dredging work underway in the San Juan River, an official source declared on 24 October. The military deployment seeks “to strike a blow at drug trafficking in this area” along the border, army spokesperson Col. Juan Morales said in statements published on the official website “19 de julio” [19 July], confirming statements along the same lines made on 23 October. The operations are being carried out in coordination with the police and the navy – which will patrol the San Juan River, which runs along the northeastern border with Costa Rica until emptying into the Caribbean Sea – following indications that drug traffickers were planning to establish a route there for the shipment of drugs to the north. “There is a naval component that is pursuing the sovereignty missions, both at the entrance to the sea and on the river itself,” while another component is reinforcing the elements operating on land, Morales said. The army increased its presence on the border on 23 October, with an unspecified number of personnel added to the more than three hundred it had in the area, a day after San José ordered a police mobilization along the border with Nicaragua. San José ordered the deployment after complaining, in a protest note sent to Managua, that a Nicaraguan dredger was throwing sediment extracted in dredging the San Juan River onto the Costa Rican bank and that military personnel had trespassed on its territory, violating its sovereignty. “As a sovereign state, Costa Rica has the full right to do what is appropriate for it as a country,” and “in the same way, we as a sovereign country also have the full power to do what is appropriate for us,” Morales affirmed. By Dialogo October 26, 2010
Maybe you’re feeling overwhelmed with your to-do list within your marketing department, and think it’s time to hire an outside digital marketing agency? Maybe you’re wearing “multiple hats” and need a little help running campaigns and revamping your overall marketing strategy?Whatever the reason is, make sure you know what to look for when hiring a digital marketing agency, and be aware of what key factors will set some agencies apart from others. Before you sign a contract, here are 5 questions to ask a digital marketing agency:Question 1: How Will You Help Me Meet My Marketing Goals?A good agency should start with your growth goals for the year and then create a strategy to help your organization move step by step toward meeting those goals. It is always important to make sure you’re strategy contains SMART goals – Specific, Measureable, Attainable, Relevant and Timely. continue reading » 23SHARESShareShareSharePrintMailGooglePinterestDiggRedditStumbleuponDeliciousBufferTumblr
A further 32 games were scheduled to be played during the second international break on two match days on June 4 and 9.FIFA added that the matches could still go ahead if the member associations due to play each other mutually agree and safety standards are met, subject to prior approval from both FIFA and the AFC.It added that upcoming qualifiers for the Olympic Football Tournaments were likely to be played as scheduled, with the exception of thewomen’s Olympic qualifiers playoff between South Korea and China.The playoff will now take place during the June 1-10 international window.FIFA and the AFC also announced new dates for the AFC Futsal Championship Turkmenistan, which was scheduled for Feb. 26-March 8 and will now be held from August 5-16. Topics : FIFA and the Asian Football Confederation (AFC) have agreed to postpone the Asian World Cup qualifying matches in March and June due to the coronavirus outbreak, the world soccer body said on Monday.”Following consultation with Asia’s member associations, FIFA and the Asian Football Confederation (AFC) have agreed to postpone the upcoming Asian qualifiers for the FIFA World Cup Qatar 2022,” FIFA said in a statement.China’s matches against Maldives at home and Guam away were already moved to Buriram, Thailand and were set to be played in an empty stadium, but more than two dozen other matches were scheduled around the continent on two match days on March 26 and 31.